{"id":8839,"date":"2019-09-05T13:30:31","date_gmt":"2019-09-05T18:30:31","guid":{"rendered":"http:\/\/www.sector67.org\/blog\/?p=8839"},"modified":"2019-09-05T13:30:33","modified_gmt":"2019-09-05T18:30:33","slug":"july-2019-workshop-code-with-python-and-edit-bacteria-using-crispr-cas","status":"publish","type":"post","link":"https:\/\/www.sector67.org\/blog\/2019\/july-2019-workshop-code-with-python-and-edit-bacteria-using-crispr-cas\/","title":{"rendered":"July 2019 Workshop – Code with Python and edit bacteria using CRISPR-Cas"},"content":{"rendered":"

A bioengineering workshop was conducted at Sector67 on July 15 & 18 – 2019, that bridged concepts in genome editing and computer programming. In this hands-on workshop, students edited a bacterial genome using the new and exciting CRISPR-Cas9 system and performed string manipulations using Python to visualize and code the cellular editing process.<\/p>

\"\"<\/figure>

In the wet-lab part of this workshop, students prepared Petri plates to grow bacteria.\u00a0 The cultured bacteria were then incubated with multiple components constituting the CRISPS-Cas9\u00a0 system (http:\/\/www.the-odin.com\/diy-bacterial-crispr-refill-classroom-kit\/<\/a>)\u00a0 to edit the genome. \u00a0 The cells were then grown on Petri plates containing a growth medium that selects for the edited cells.\u00a0 <\/p>

\"\"<\/figure>

In the computer lab the students learnt basic string manipulation code in Python to identify a target sequence in a genome by scanning, a process perhaps similar to the mechanism of CRISPR-Cas9.\u00a0 <\/p>

The class was capped at eight students and this allowed students to perform both the wet-lab experiments and spend time on the desktop computers to learn and code in Python.\u00a0 The workshop was spread over two 4-hour days, July 15 and July 18. The genome editing experiment was conducted on the first day and the Petri plates were incubated for cell growth. <\/p>

\"\"<\/figure>
\"\"<\/figure>

   The students came back the second day to look at the results and learn to code.<\/p>

\"\"<\/figure>

All eight students had  success in their editing experiments !<\/p>

\"\"<\/figure>
\"\"<\/figure>
\"\"

<\/figcaption><\/figure>
\"\"<\/figure>

Stay tuned to more classes and workshops along these lines.\u00a0<\/p>","protected":false},"excerpt":{"rendered":"

A bioengineering workshop was conducted at Sector67 on July 15 & 18 – 2019, that bridged concepts in genome editing and computer programming. In this hands-on workshop, students edited a bacterial genome using the new and exciting CRISPR-Cas9 system and…<\/span><\/p>\n

Read more ›<\/a><\/div>\n

<\/p>\n","protected":false},"author":732,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[610,608,609],"class_list":["post-8839","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-bacterial-genome-editing","tag-crispr-cas9","tag-python"],"_links":{"self":[{"href":"https:\/\/www.sector67.org\/blog\/wp-json\/wp\/v2\/posts\/8839","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.sector67.org\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.sector67.org\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.sector67.org\/blog\/wp-json\/wp\/v2\/users\/732"}],"replies":[{"embeddable":true,"href":"https:\/\/www.sector67.org\/blog\/wp-json\/wp\/v2\/comments?post=8839"}],"version-history":[{"count":7,"href":"https:\/\/www.sector67.org\/blog\/wp-json\/wp\/v2\/posts\/8839\/revisions"}],"predecessor-version":[{"id":8852,"href":"https:\/\/www.sector67.org\/blog\/wp-json\/wp\/v2\/posts\/8839\/revisions\/8852"}],"wp:attachment":[{"href":"https:\/\/www.sector67.org\/blog\/wp-json\/wp\/v2\/media?parent=8839"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.sector67.org\/blog\/wp-json\/wp\/v2\/categories?post=8839"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.sector67.org\/blog\/wp-json\/wp\/v2\/tags?post=8839"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}